Study on Callus Induction and Plant Regeneration of Lycoris radiate Herb.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Callus Induction and Plant Regeneration of Lycoris radiate Herb.

Author(s):: LIU He-xia; ZHOU Jian; Institute of Forest Resources and Environment，Nanjing Forestry University，Nanjing，Jiangsu 210037

Abstract:: Taking Lycoruis radiate Herb. as material，the effect of some factors such as various hormone concentration，different parts of Lycoruis radiate Herb. on callus induction and plant regeneration were studied.The results showed that the explants selection was the most significant factor of callus induction，and the seed embryos was the best explants for callus induction.The optimal mediums for the callus induction was MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L and the rate reached 82.4%.In the subsequent callus further training，the optimal mediums for the callus induction was MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L，the optimal mediums for the callus induction was NAA 0.1 mg/L.The best matrix for Lycoris radiata regeneration plants was humus and perlite in the proportion of 1∶1 composition，with survival rate 70%.

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Last Update: 2014-08-23