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Study on Observation of Aseptic Germination of Bletilla striata Seeds and Rapid Propagation of Bletilla bulbocodioides (PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年03期
Page:
158-160
Research Field:
Publishing date:

Info

Title:
Study on Observation of Aseptic Germination of Bletilla striata Seeds and Rapid Propagation of Bletilla bulbocodioides
Author(s):
ZHANG Yan; LI Bin; LI Ru-juan; LI Si-feng
Xi’an Botanic Garden，Shaanxi Institute of Botany,Xi’an, Shaanxi 710061
Keywords:
Bletilla; aseptic germination; rapid propagation
PACS:
S 682.1+9
DOI:
-
Abstract:
Taking the seeds of Bletilla bulbocodioides as material, the protocom-like-body development in the aseptic germination of Bletilla seeds and tissue culture and rapid propaation of Bletilla were studied using protocorm and leaves as explants. The results showed that during the process of seed germination,two kinds of globules growth into seedling were discovered:the globules grew into seedlings through their protocorms;the globules grew first into rhizome, then into seedling. The optimum medium for PLB induction was 1/2MS+6-BA 5.0 mg/L+NAA 0.2 mg/L,and the proliferation multiple was 2.71.The tissue culture plantlets were not obtained when using the leaves as explants.

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Last Update: 2014-08-23