Taking the young leaves，the tender stem segments,the half lignified stem segments,the leaf buds,the buds and the seeds of Camellia chungtsoensis S.Y.Liang et L.D.Huang as explants，with 75% alcohol，0.1% HgCl2，0.2% HgCl2 and NaClO4 kinds of sterilization agent for sterilization， explants in tissue culture of Camellia chungtsoensis S.Y.Liang et L.D.Huang optimal sterilization program were studied.The results showed that it was easy to the young leaves and the seeds to disinfect.The better disinfection condition for the young leaves was 75% alcohol sterilized for 30 s,0.1% HgCl2 of mercury disinfected for 5 minutes and 0.1% HgCl2 of mercury disinfected for 5 minutes again.The better disinfection condition for the seeds was 75% alcohol sterilized for 40 s，0.1% HgCl2 of mercury disinfected for 20 to 30 minutes and then 0.2% HgCl2 of mercury disinfected for 20 minutes.It wasn’t easy to the stems to disinfect，the better way was time period of disinfection.The better disinfection condition for the young stems was 75% alcohol sterilized for 25 s，0.1% HgCl2 of mercury disinfected for 10 minutes and then 0.2% HgCl2 of mercury disinfected for 5 minutes.The better disinfection condition for the half lignified stems was 75% alcohol sterilized for 35 s，0.1% HgCl2 of mercury disinfected for 12 minutes and then 0.2% HgCl2 of mercury disinfected for 6 minutes.And difficult to the buds.It was difficult to achieve the desired effect of time period of disinfection with NaClO+HgCl2 and successive strip the outer bracts sterilization method.