The Prunus sibirica was used as material，the kind of basic medium，the kind of explants and the different proportions of hormones impact the growth condition of explants in the various stages of tissue culture were studied.The results showed that the best sterilization effect was used HgCl2 1 g/L as disinfectants to disinfect 3 min；the division and growth condition of explants was better in improved MS[C(NH4+)∶C(NO3-)=0.16]medium；the most suitable explants was shoot tips；the most suitable proportions of hormones was 6-BA 1.0 mg/L and NAA 0.05 mg/L in primary culture；the most suitable proportions of hormones was 6-BA 0.5 mg/L and NAA 0.1 mg/L in subculture.