Study on Optimum for Regeneration System of Leaves in Hovenia acerba(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Optimum for Regeneration System of Leaves in Hovenia acerba

Author(s):: SUN Ye-fang¹; ZHENG Qi¹; SUN Jun-wei²; CHEN Zhen³; ZHOU Dan²; 1.Shaoxing Academy of Agriculture Sciences，Shaoxing，Zhejiang 312003；
2.Department of Life Sciences，China Jiliang University，Hangzhou，Zhejiang 310018；
3.School of Life Sciences，Taizhou University，Taizhou，Zhejiang 318000

Keywords:: Hovenia acerba; plant growth regulator; leaf disc of regeneration plants; adventitious buds induction; rooting induction

Abstract:: A regeneration protocol for Hovenia acerba were set up via organogenesis from callus derived from leaf disc of regeneration Hovenia acerba plants in vitro.The results showed that the best explants were mature leaves with dark green color，and the best placement way of explants was face-up；the most appropriate medium for the induction of callus and adventitious buds was 1/2MS+BA 2.0 mg/L+IAA 1.0 mg/L，with the induction rate more than 85% and proliferation rate more than 6.0；the most appropriate medium for the rooting was 1/4MS+NAA 0.1 mg/L，with addition of 2% sucrose，in which the rooting rate exceeded 95%.

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Last Update: 2014-08-23