Study on Regeneration of Castor Bean in vitro Using Hypocotyl Explants(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年03期

Page:

107-109

Research Field:

Publishing date:

Info

Title:

Study on Regeneration of Castor Bean in vitro Using Hypocotyl Explants

Author(s):

LIU Peng¹; 2; DONG Yong-yi¹; CHEN Yong-sheng²; 3

1.College of Agriculture，Inner Mongolia University for Nationalities，Tongliao，Inner Mongolia 028042；

2.Engineering Research Center of Castor at Universities of Inner Mogolia Autonomons，Tongliao，Inner Mongolia 028042；

3.School of Life Science，Inner Mongolia University for Nationalities，Tongliao，Inner Mongolia 028042

Keywords:

castor bean; hypocotyl; in vitro regeneration

PACS:

S 565.635.3

DOI:

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Abstract:

Taking cotyledon explants of castor bean as materials，the effect of different species and concentrations of cytokinin on shoot induction and different concentrations of auxin on root induction were studied.The results showed that mature seed-derived hypocotyl explants produced adventitious shoots when placed on MS medium containing 0.5 mg/L 6-BA or 10.0 mg/L ZT.The rate of shoot regeneration was maximal (86.7% and 68.9% respectively).Shoots were transferred to root induction medium (MS+0.2 mg/L NAA)，which root regeneration frequency achieved 26.7%.

References:

[1]Anjani K.Castor genetic resources：A primary gene pool for exploitation[J].Industrial Crops and Products,2012,35(1):1-14.

[2]Birch R G.Plant transformation：problems and strategies for practical application [J].Ann Rev Plant Physiol Plant Mol Biol 1997,48:297-326.

[3]Sujatha M，Sailaja M.Stable genetic transformation of castor (Ricinus communis?L.) via Agrobacterium tumefaciens-mediated gene transfer using embryo axes from mature seeds[J].Plant Cell Rep,2005,23:803-810.

[4]Malathi B，Ramesh S，Rao K V，et al.Agrobacterium-mediated genetic transformation and production of semilooper resistant transgenic castor (Ricinus communis L.)[J].Euphytica,2006,147:441-449.

[5]Ahn Y J，Chen G Q.In vitro?regeneration of castor (Ricinus communis?L.) using cotyledon explants[J].Hort Science,2008,43(1):215-219.

[6]Sujatha M，Reddy T P.Differential cytokinin effects on the stimulation of in vitro shoot proliferation from meristematic explants of castor (Ricinus communis?L.)[J].Plant Cell Rep,1998(7):561-566.

[7]张利明,侯玲玲,李文彬,等.蓖麻组织培养和植株再生的研究[J].中国油料作物学报,2009,31(2):253-255.

[8]Alam I，Sharmin S A，Mondal S C，et al.?In vitro?micropropagation through cotyledonary node culture of castor bean (Ricinus communis?L.)[J].Australian Journal of Crop Science,2010,4(2):81-84.

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