Study on Stem Apex Detoxification of Siraitia grosvenorii(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: WU Qun-ying¹; LI Bo-lin²; LI Jing-yun³; 1.Guilin Medical University，Guilin，Guangxi 541004；
2.Guangxi Normal University，Guilin，Guangxi 541004；
3.Guangxi Traditional Chinese Medical University，Nanning，Guangxi 530001

Keywords:: Luohanguo mosaic virus (WMV-2-Luo); stem apex detoxification; ELISA

Abstract:: Siraitia grosvenorii in vitro was used as material in this experiment to eliminate Luohanguo mosaic virus (WMV-2-Luo) by combing tissue culture of shoot-tip with heat therapy，and detected by indirect ELISA method and electron microscope.The results showed that the suitable time for heat treatment was 7~15 days，the survival rate of tissue culture seedling was up to 90%~100%；0.2~0.5 mm shoot-tip with heat treatment by 38.5℃ for 10 days of Siraitia grosvenorii were 100% free from WMV-2-Luo.

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Last Update: 2014-08-23