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Codon Optimization and Expression of MAP30 in Pichia pastoris Expression System(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年24期
Page:
112-115
Research Field:
Publishing date:

Info

Title:
Codon Optimization and Expression of MAP30 in Pichia pastoris Expression System
Author(s):
WANG Fang; WANG Li-yuan; QIAO Yu; HAN Xu; DING Guo-hua
Key Laboratory of Plant Biology of Heilongjiang Province,College of Life Science and Technology,Harbin Normal University，Harbin,Heilongjiang 150025
Keywords:
Momordica charantia; MAP30; Pichia pastoris; pGAPHα; optimized gene
PACS:
S 642.5
DOI:
-
Abstract:
Taking Momordica charantia as experimental material，the codon optimized MAP30 gene of Momordica charantia in Pichia pastoris expression was studied by SDS-PAGE and Western-blot.The results showed that the expression quantity of the optimized MAP30 gene was increased slightly in the neotype Pichia pastoris expression system.pGAPHα vector composed of GAP promotor was safer than pPIC9 composed of AOX promotor.It is supposed that the new way should be used in medical field.

References:

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Last Update: 2014-12-08