Taking the stem segments of Dracaena godseffiana as explant，the callus induction and plantlet regeneration were studied，in order to select the suitable hormone combination in the process of tissue culture.The results indicated that：the optimum medium for callus induction was MS+6-BA 2.0 mg/L+TDZ 0.5 mg/L+2,4-D 1.0 mg/L+sucrose 30 g/L+agar 6 g/L，induction rate was 88.9%；the optimum medium for adventitious bud differentiation was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L+sucrose 30 g/L+agar 6 g/L，induction rate was 59.26%；The optimum medium for adventitious bud proliferating was MS+6-BA 1.0 mg/L+NAA 0.8 mg/L+GA3 0.2 mg/L+sucrose 30 g/L+agar 6 g/L，proliferation coefficient was 1.91；The optimum medium for rooting was 1/2MS+IBA 1.0 mg/L+sucrose 15 g/L+agar 6 g/L，rooting rate was 81.2%.