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Tissue Culture of Stem Tip and Rapid Ppropagation of (Fragaria×ananassa Duch.‘Benihoppe’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年24期
Page:
106-108
Research Field:
Publishing date:

Info

Title:
Tissue Culture of Stem Tip and Rapid Ppropagation of (Fragaria×ananassa Duch.‘Benihoppe’
Author(s):
DONG Jing-chao
Liaoning Institute of Sandyland Improvement and Utilazation，Fuxin,Liaoning 123000
Keywords:
Fragaria×ananassa Duch ‘Benihoppe’; stem tip; tissue culture; rapid propagation
PACS:
S 668.4
DOI:
-
Abstract:
Taking the Fragaria×ananassa Duch ‘Benihoppe’ as the experimental material，the shoot tip as explants，MS as the basic medium，the optimal proportion of hormones and best transplanting substrate on growth of strawberry plantlets confidante were studied，in order to establish an efficient system of rapid propagation technique of cultivating a tip of stem tissue.The results showed that the suitable medium for primary MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+agar 5 g/L，germination rate was 81%；suitable proliferation culture medium for MS+6-BA 0.25 mg/L+NAA 0.1 mg/L+sucrose 30 g/L+agar 5 g/L，mutiplication coefficent was 9.1.The most suitable rooting medium was 1/2 MS+NAA 0.1 mg/L+sucrose 30 g/L+agar 5 g/L，the rooting rate was 98.00%.The best transplanting matrix∶nutrition soil∶perlite∶vermiculite= 1∶1∶1，transplanting survival rate reached 97.7%.

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Memo

Memo:
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Last Update: 2014-12-08