|Table of Contents|

Initial Screening of Insect Resistant Gene to Leptocybe invasa Fisher& La Salle(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年24期
Page:
88-91
Research Field:
Publishing date:

Info

Title:
Initial Screening of Insect Resistant Gene to Leptocybe invasa Fisher& La Salle
Author(s):
WANG Wei1; XU Jian-min2; LI Guang-you2; HAN Chao2; WU Shi-jun2; LU Zhao-hua2
1.Guangzhou Institute of Landscaping Gardening,Guangzhou,Guangdong 510405
2.Research Institute of Tropical Forestry,Chinese Academy of Forestry,Guangzhou,Guangdong 510520
Keywords:
Leptocybe invasa Fisher&;  La Salle; insect resistant gene; transcriptome sequencing; Real-time PCR
PACS:
S 718
DOI:
-
Abstract:
The high-sensitive strain of Eucalyptus ‘DH201-2’ (sample A) and high-resistant strain of Eucalyptus urophylla ‘A107’ (sample B) were chosen for transcriptome sequencing,971 differential expression sequences were screened through de novo assembly and differential expression analysis for sequence.19 target genes were chosen to make RT-PCR.The results showed that the target gene had a good specificity amplification except the target gene 16.The average amount of expression of G1,G5,G9,G10,G18 and G19 in sample B was significant higher than in sample A.The average amount of expression of G3,G4 and G11 in sample A was significantly higher than in sample B.There were no significant differences of the average amount of expression among the last target gene.Target gene G1,G3,G4,G5,G9,G10,G11,G18,G19 maybe the susceptibility gene to insect resistant gene to Leptocybe invasa Fisher& La Salle.

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Last Update: 2014-12-08