|Table of Contents|

Effects of Phytohormones on Callus Formation and Buds Differentiation of Cucumber(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年24期
Page:
84-87
Research Field:
Publishing date:

Info

Title:
Effects of Phytohormones on Callus Formation and Buds Differentiation of Cucumber
Author(s):
WANG Ping1; SHEN Xiao-ting2; WANG Gang3; JI Jing3
1.School of Marine Science and Technology，Huaihai Institute of Technology，Lianyungang，Jiangsu 222005；
2.Gulin County Vocational High School，Gulin，Sichuan 646500；
3.School of Environmental Science and Engineering，Tianjin University，Tianjin 300072
Keywords:
cucumber; phytohormones; cotyledonary nodes; tissue culture
PACS:
S 642.2
DOI:
-
Abstract:
Using cotyledonary nodes of ‘Jinyan 4’ as explants，the orthogonal experiment design L9(34) was applied to investigate the effects of phytohormones combination on differentiation and elongation of buds with MS basic medium plus 4 phytohormones (6-BA,IAA,ABA,KT) in cucumber.The results showed that MS medium plus any three of four phytohormones could form callus，MS medium plus 1~2 mg/L 6-BA，0.5 mg/L ABA and 4 mg/L KT (or 0.050 mg/L IAA) could facilitate the differentiation of adventitious buds obviously in cucumber，whereas，MS medium plus 2 mg/L 6-BA，0.025 mg/L IAA and 4 mg/L KT was beneficial for the elongation of buds.The effects of 6-BA，IAA，ABA on regeneration rates，number of buds and length of buds were similar and more evident than those of KT when the explants were cultured for 2 weeks.Among these 4 phytohormones，6-BA and ABA were demonstrated to be more effective on regeneration rates，number of buds and length of buds relatively when the explants were cultured for 6 weeks.Although ABA could promote of the differentiation of adventitious buds in cucumber，it could suppress the elongation of buds when the explants were cultured for 6 weeks.

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Last Update: 2014-12-08