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Research on Micropropagation Technology of Aspidistra elatior(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年24期
Page:
80-83
Research Field:
Publishing date:

Info

Title:
Research on Micropropagation Technology of Aspidistra elatior
Author(s):
XIE Li; ZHOU Huan; ZHANG Zhi-sheng; HE Yi-mo; GUO He-rong
1.Guangdong Provincial Key Laboratory of Plant Molecular Breeding，South China Agricultural University，Guangzhou,Guangdong 510642
Keywords:
Aspidistra elatior; rapid propagation; callus; induction; differentiation; root differentiation
PACS:
Q 81
DOI:
-
Abstract:
Using young leaves of Aspidistra elatior as explant,the induction，bud differentiation and root differentiation of the callus of Aspidistra elatior were studied.The results showed that the basic medium，inoculation manner，size of explant，plant growth regulator，citric acid and active carbon significantly influenced the induction of callus.The induction rate was highest(31.25%) when leaf segments of 0.5 cm2were horizontally inoculated on the medium of 1/2 MS+TDZ 3.00 mg/L+NAA 0.10 mg/L+citric acid 0.10 g/L for 45 days.Culture method，cutting treatment，TDZ and the number of subculture significantly affected the differentiation rate of bud.The bud differentiation rate was the highest(114.81%) when callus cultured in 1/2 MS+TDZ 3.00 mg/L+NAA 0.10 mg/L+citric acid 0.1 g/L.NAA and active carbon significantly influenced root differentiation.When the buds cultured in 1/2 MS+6-BA 0.10 mg/L+NAA 1.00 mg/L+active carbon 1.00 mg/L for 30 days，the root differentiation rate was 39.26%.

References:

[1]王元军.观叶植物蜘蛛抱蛋的栽培[J].北方园艺,2005(1):7.
[2]许桂芳.7种观赏植物对甲醛的净化效果及生理响应[J].中国农学通报,2012,28(19):266-269.
[3]Dingle P，Tapsell P，Hu S.Reducing Formaldehyde Exposure in Office Environments Using Plants[J].Bull Environ Contam Toxicol,2000，64(2):302-308.
[4]冯青,高群英,张汝民,等.3种百合科植物挥发物成分分析[J].浙江农林大学学报,2011,28(3):513-518.
[5]周勇,雷强军,刘良宏.一叶兰的组织培养和快速繁殖[J].科协论坛，2008(7):72.
[6]谭文澄,戴策刚.观赏植物组织培养技术[M].北京:中国林业出版社，1997:150-156，234-241.
[7]黄磊,陈之林,吴坤林,等.切割方式和外植体大小对蝴蝶兰叶片诱导类原球茎的影响[J].热带亚热带植物学报,2009,17(3)：261-266.
[8]唐君,赵冬兰,刘亚菊.外植体对甘薯茎尖培养与植株再生的影响[J].中国农学通报,2004,20(4):121-122,131. [9]Nawab A，Robert M S，Walter E S.Regeneration of Cucumis sativus from cotyledons of small explants [J].Hort Sci，1991，26(7)：925-927.
[10]孙志栋,陈惠云,葛红,等.中国春兰组织培养初探[J].安徽农学通报,2006,12(1)：20-21.

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Last Update: 2014-12-08