|Table of Contents|

In vitro Regeneration of Pepper via Rosettes from Cotyledons with Petioles(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年23期
Page:
122-126
Research Field:
Publishing date:

Info

Title:
In vitro Regeneration of Pepper via Rosettes from Cotyledons with Petioles
Author(s):
ZANG Shun; CAI Xia
Key Laboratory of Horticulture Science for Southern Mountainous Regions，Ministry of Education，Key Laboratory of Olericulture，College of Horticulture and Landscape Architecture，Southwest University，Chongqing 400715
Keywords:
pepper; cotyledons with petioles; rosettes; elongated shoots; orthogonal experiment
PACS:
S 641.3
DOI:
-
Abstract:
Cotyledons with petioles of ‘Qianjiao No.4’ pepper (Capsicum annuum) were used as explants which differentiated into rosettes.The influences of BA，IAA and AgNO3 on cotyledon explants differentiation were tested according orthogonal experiment design.The results showed that the influences of BA，IAA and AgNO3 on explants differentiation were significant at 5% level and ranked as BA>AgNO3>IAA.MS+BA 6.0 mg/L+IAA 1.0 mg/L+AgNO3 6.0 mg/L+sugar 30 g/L+agar 7 g/L was chosen as the best medium for cotyledon explants differentiation with 〖JP4〗differentiation rate as 82%.After four weeks culture on MS/MB+BA 6.0 mg/L+IAA 0.5 mg/L+GA3 (0，1，2，3 mg/L)+sugar 30 g/L+agar 7 g/L，the elongated shoots from rosettes were obtained on elongation media.GA3 was indispensable for obtainment of elongated shoots，and its concentration (1，2，3 mg/L) affected the elongation rates at 1% level and affected the average number of elongated shoots at 5% level.The MB basal medium was not better than MS basal medium at the elongation rate and the average number of elongated shoots.But the MB basal medium gave more well-grown shoots than the MS basal medium.MB+BA 6.0 mg/L+IAA 0.5 mg/L+GA3 2 mg/L+sugar 30 g/L+agar 7 g/Lwas chosen as the best elongation medium with elongation rate as 36%.On rooting medium，the elongated shoots rooted well and developed into plants.

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Last Update: 2014-08-26