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SCAR Marker and Application of Resistant Gene Ty-3 of Tomato Yellowing Leaf Virus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年23期
Page:
119-122
Research Field:
Publishing date:

Info

Title:
SCAR Marker and Application of Resistant Gene Ty-3 of Tomato Yellowing Leaf Virus
Author(s):
DONG Pan-pan1；ZHAO Mei-ai 2; 3; 4；GUAN Cong2；WANG Fu1；WANG Hui1
1.College of Horticulture，Qingdao Agricultural University，Qingdao，Shandong 266109
2.College of Life Sciences，Qingdao Agricultural University，Qingdao，Shandong 266109
3.Qingdao Key Lab of Germplasm Innovation and Application of Major Crops，Qingdao，Shandong 266109
4.Key Lab of Plant Biotechnology in Universities of Shandong，Qingdao，Shandong 266109
Keywords:
TYLCV; SCAR; Ty-3; seedling identification; field identification
PACS:
S 641.2
DOI:
-
Abstract:
Taking 49 tomatos as materials，5 resistant homozygous lines (Ty-3/Ty-3)and 5 homozygous susceptible lines (ty-3/ty-3) were amplified by specific primers 25 generations self-lines and 14 F1 hybrids were amplified using this marker，at the same time these lines were well validated in the field by nature infection.The results showed that the resistant homozygous lines produced about 630 bp PCR fragment，the susceptible genotypes could produce fragment about 320 bp.This marker could distinguish resistant and susceptible lines，and it was a co-dominant marker tightly linked to Ty-3 gene.Among 25 generations self-lines，the genotype of 4 generations self-lines were Ty-3/Ty-3，7 were Ty-3/ty-3 and 14 were  ty-3/ty-3.14 F1 hybrids were amplified by Ty-3 primer，the genotype of 4 F1 hybrids were Ty-3/Ty-3,5 were Ty-3/ty-3 and 5 F1 hybrids didn’t contain Ty-3 gene.These lines were well validated in the field by nature infection and the disease incidence was 78.6% coincident with the result of PCR method.In the TYLCV resistance breeding，PCR was a rapid screening method to improve breeding efficiency.

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Last Update: 2014-08-26