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In silico Cloning and Bioinformatics Analysis on HY5 Gene of  Brassica napus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年23期
Page:
116-118
Research Field:
Publishing date:

Info

Title:
In silico Cloning and Bioinformatics Analysis on HY5 Gene of  Brassica napus
Author(s):
GUO Ji-ping 1; 2
1.Department of Life Sciences，Hengshui College，Hengshui，Hebei 053000
2.College of Food Science and Engineering，Harbin Institute of Technology，Harbin,Heilongjiang 150090
Keywords:
HY5; Brassica napus; photoreceptor; in silico cloning
PACS:
S 634.3
DOI:
-
Abstract:
With HY5 gene in Brassica rapaas probe,the cDNA of HY5 gene of Brassica napus  was in silico cloned.Using bioinformaticstool,amino acid sequence coded by HY5of Brassica napus was predicted and homology compared with Arabidopsis thalianaand Brassica rapa.Its phylogenetic tree was constructed.The results showed that HY5 of  Brassica napus coded a protein of 164 amino acids.The calculated molecular weight of it was 18 0378 Da.It was a hydrophilic protein.The homology of  HY5 protein between Brassica napus and Brassica rapa was higher than that between Brassica napus and Arabidopsis thaliana.The HY5gene coding sequence of  Brassica napus was cloned preliminary，and laid the foundation for further study its function.

References:

[1] Wu D，Hu Q，Yan Z，et al.Structural basis of ultraviolet-B perception by UVR8[J].Nature,2012，484(7393):214-219.
[2] Rizzini L,Favory J J，Cloix C，et al.Perception of UV-B by the  Arabidopsis UVR8 protein[J].Science，2011，332:103-106.
[3] Oyama T，Shimura Y，Okada K.The Arabidopsis HY5 gene encodes a bZIP protein that regulates stimulus induced development of root and hypocotyl[J].Genes and Development，1997(11):2983-2995.
[4] 惠婕，杜静，黄丛林，等.植物光敏色素研究进展[J].北方园艺，2010(7)：203-205.
[5] Ang L H，Deng X W.Regulatory hierarchy of photomorphogenic loci：allele-specific and light-dependent interaction between the HY5 and COP1 loci[J].Plant Cell，1994(6):613-628.
[6] Foster R，Izawa T，Chua N.Plant bZIP proteins gather at ACGT elements[J].Faseb Journal，1994(8):192-200.

Memo

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Last Update: 2014-08-26