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Methodology Investigation of Reversal HPLC Measuring the Content of  Fruit Polyphenol in Hawthorn(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年23期
Page:
106-108
Research Field:
Publishing date:

Info

Title:
Methodology Investigation of Reversal HPLC Measuring the Content of  Fruit Polyphenol in Hawthorn
Author(s):
GAO Xiu-yan；ZHAO Yu-hui；GUO Yin-shan；LIU Zhen-dong；DONG Wen-xuan；LV De-guo
College of Horticulture，Shenyang Agricultural University，Shenyang,Liaoning 110866
Keywords:
hawthorn; fruit polyphenol; HPLC
PACS:
S 661.5
DOI:
-
Abstract:
With hawthorn as material,by reverse phase high performance liquid chromatography (HPLC)，method of detection of various polyphenol content in hawthorn fruit were establised.The results showed that the high performance detection system was as follows：chromatographic column was Agilent ZORBAX Extend-C18 colume (250 mm×4.6 mm，5 μm)；moving phase was acetonitrile-0.5% phosphoric acid solution；gradient elution：0～9 min（18∶82～20∶80），9～25 min（20∶80～50∶50），25～33 min（50∶50～100∶0）；testing wavelength was 280 nm；column temperature was 30℃；flow velocity was 09 mL/min，injection volumn was 20 μL.This method can determine flavone and procyanidine contents in hawthorn fruit simultaneously under the same condition.According to the accuracy test，repeatability test，stability test it and recovery test indicated that the previously established sample solution preparation method was also suitable for procyanidine abstraction.

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Last Update: 2014-08-26