|Table of Contents|

Isolated Microspore Culture of Spring-white Radish  (Raphanus sativus  L.var.  longpinnatus  Bailey)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年23期
Page:
31-33
Research Field:
Publishing date:

Info

Title:
Isolated Microspore Culture of Spring-white Radish  (Raphanus sativus  L.var.  longpinnatus  Bailey)
Author(s):
ZHANG Li；ZHENG Peng-jing
Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences，Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China)，Ministry of Agriculture，Beijing 100097
Keywords:
radishradishradish' target='_blank'> FONT-SIZE: 10.5pt">; microspore culturemicrospore culture' target='_blank'> FONT-SIZE: 10.5pt">; cold pretreatmentcold pretreatment' target='_blank'> FONT-SIZE: 10.5pt">; activated charcoalmicrospore culture' target='_blank'> FONT-SIZE: 10.5pt">activated charcoalmicrospore culture; cold pretreatment; activated charcoal
PACS:
S 631.1
DOI:
-
Abstract:
To establish a spring-white radish microspore culture system，taking 9 spring-white radish cultivars as materials,the effects of genotype on embryoblast rate of isolaled microspone in spring-white radish and cold pretreatment for flower buds and medium added with activated charcoal on inducing microspore embryogenesis were studied.The results showed that,microspore embryogenesis were induced among which ‘Dashi’ had the highest microspore embryogenesis rate,reached 23 embryo per 30 anthers.A significant improvement of embryogenesis was achieved by using cold pretreatments and medium with 100 mg/L activated charcoal,while single treatment of cold pretreatment and charcoal had no significant effect.There were differences of suitable cold pretreatments time among different varieties.

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Last Update: 2014-08-11