Preliminary Study on the Characteristics of Endogenous Inhibitory Substances for Walnut(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Preliminary Study on the Characteristics of Endogenous Inhibitory Substances for Walnut

Author(s):: JIA Cai-xia¹；ZHAI Mei-zhi¹；LIN Lin-tian²；ZHANG Tan¹; 1.College of Forestry，Northwest Agriculture and Forestry University，Yangling，Shaanxi 712100
2.Yangling Vocational and Technical College，Yangling，Shaanxi 712100

Keywords:: walnut; inner inhibitory substances; paper chromatography; R_f value; nut soaking in warm water

Abstract:: Taking walnut endocarps and seeds (seed coats，cotyledons and embryo) as materials，the effect of endogenous inhibitors on seeds germination and seeding growth of Chinese cabbage and wheat were studied，and the two methods of removing inner inhibitory substances that were soaking in warm water and washing with flowing water were compared.The results showed that there were endogenous inhibitors with higher activities in walnut seeds.And increasing of the concentration of endogenous inhibitors would enhance the inhibitory effect.The inner inhibitory substances from kernels showed stronger inhibitory effect than endocarps.The highest zone of paper chromatography of ether extraction of endogenous inhibitory substances was R_f1.0；the highest zones of paper chromatography of remained liquid after extraction with ether were R_f0.1 and R_f0.9.This demonstrated that endogenous inhibitors of walnut seeds consisted of not only ingredient easily soluble in water，but also those easily soluble in non-polar ether solvent.And water dissolved inner inhibitors showed stronger inhibitory effect on seeds germination and seeding growth.The endogenous inhibitory substances could be partly removed by soaking in warm water (40℃) or washing with flowing water.

[1] 郗荣庭,张毅萍.中国果树志·核桃卷[M].北京:中国林业出版社,1995:1-18.

[2] 郝艳宾,刘军,刘佳梦.核桃栽培技术问答[M].北京:中国农业出版社,1998：1-7,12-15.

[3] 张开春.果树育苗手段[M].北京:中国农业出版社,2000:2-6.

[4] 贾彩霞,翟梅枝,宋艳枝.核桃壳内源抑制物质活性的初步研究[J].西北林学院学报,2006,20(3):98-100.

[5] 曲芬霞,姚霞珍,郭建斌,等.西藏核桃休眠及萌发特性研究[J].种子,2011,30(3):44-46.

[6] 王炎,赵敏,俞佳林.北五味子种子休眠特性及内源抑制物质的研究[J].中国中药杂志,1997,22(1):10-14.

[7] 赵敏,王炎,康莉.刺五加果实及种子内源萌发抑制物质活性的研究[J].中国中药杂志,2001,26(8):534-537.

[8] 赵敏,王炎,张伟.北五味子内源抑制物质活性的消长规律[J].东北林业大学学报,1999,27(3):44-47.

[9] 赵敏,王炎,张伟.北五味子内源抑制物质特性的初步研究[J].东北林业大学学报,1999,27(5):62-64.

[10]赵敏,徐兆飞,王荣华,等.桔梗种子内源抑制物质特性的初步研究[J].东北林业大学学报,2000,28(1):51-54.

[11]孙佳,郭江帆,魏朔南.植物种子萌发抑制物质物质概述[J].种子,2012,31(4):57-61.

Last Update: 2014-08-10