Study on Adventitious Roots Induction and Proliferation Culture of Pseudostellaria heterophylla(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年02期

Page:

147-149

Research Field:

Publishing date:

Info

Title:

Study on Adventitious Roots Induction and Proliferation Culture of Pseudostellaria heterophylla

Author(s):

LIANG Yu-yong¹; LIAO Ling¹; ZUO Bei-mei²; GAO Wen-yuan²; 3

1.Tongren Polytechnic，Tongren，Guizhou 554300；

2.Tianjin University of Science and Technology，Tianjin 300457；

3.Tianjin University，Tianjin 300000

Keywords:

Pseudostellaria heterophylla; adventitious roots; induction; proliferation

PACS:

S 567.5+3

DOI:

-

Abstract:

Taking Pseudostellaria heterophylla seedling as materials，different kinds and different concentrations of auxin were optimized after inducing adventitious roots，and the adventitious roots induction and proliferation culture of Pseudostellaria heterophylla were studied.The results showed that the best media for adventitious roots induction was MS+3.0 mg/L IBA+0.5 mg/L KT+0.2 mg/L NAA；in the process of adventitious roots induction，500 mL erlemeyer flask shake culture had the most proliferation multiple，with 75.90；5 L bubble reactor for more oxygen value-added had the best effect on the growth of adventitious roots，and could reach 141.50 g.

References:

[1]凌一揆,颜正华.中药学[M].北京:上海科学技术出版社,1984:211.

[2]Liu X H，Chen B，Wang Y X.Preliminarystudies on pharmacological action of total saponins from Radix Pseudostellariae[J].Jiangsu Phar Clin Resea,2000,8(3):6-8.

[3]Wang Z X，Xu S X，Zhang G G，et al.Studies on chemical constituents of Pseudostellaria heterophylla(Miq.) Pax (Ⅳ)[J].J Chin Medi Chem,1992,2(3):65-67.

[4]肖尊安,熊红.不定根发生机理的研究进展[J].生物技术通报,2002(3):31.

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