Establishment of the SRAP-PCR Reaction System of Pinus contorta(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年02期

Page:

83-86

Research Field:

Publishing date:

Info

Title:

Establishment of the SRAP-PCR Reaction System of Pinus contorta

Author(s):

WANG Qian-chun¹; HUANG Xia¹; LI Guang-sen²

1.Liaoning Academy of Forestry Science，Shenyang，Liaoning 110032；

2.Liaoning Experimental Forest Farm，Wandianzi，Liaoning 113311

Keywords:

Pinus contorta; SRAP; reaction system

PACS:

S 791.256

DOI:

-

Abstract:

To establish the optimal SRAP reaction system of Pinus contorta，its genomic DNA was extracted as template and orthogonal experimental design L₁₆(4⁵) were used.Four levels of five factors (Taq polymerase，Mg²⁺，dNTPs，DNA template and primer) were optimised.The results showed that according to the amplification results，an optimal and stable SRAP-PCR system for Pinus contorta was 0.5 U Taq polymerase，2.5 mmol/L Mg²⁺，0.15 mmol/L dNTPs，60 ng DNA template and 0.2 μmol/L primer in total 20 μL reaction solution.12 pairs SRAP primer were used in this optimized SRAP-PCR system，the results showed that this system could meet the requirement for SRAP amplification of Pinus contorta.

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