Study on Pollen Vitality and Storage Capacity of Camellia japonica L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Study on Pollen Vitality and Storage Capacity of Camellia japonica L.

Author(s):: XIE Yan-feng; XU Lin; JI Xiao-mei; CHEN Wei-dong; Wuhan Forestry and Fruit Tree Research Institute，Landscape Plants Engineering Technology Research Center of Hubei Province，Wuhan，Hubei 430075

Keywords:: Camellia japonica L.; in vitro culture; pollen vitality; storage capacity

Abstract:: Taking the pollens of Camellia japonica L.as materials，the effects of pollen vitality and storage capacity in different culture mediums,different storage time of pollens and different varieties were studied.The results showed that the highest germination rate for all the seven varieties could reach or exceed 90%，and the ‘Tianshanfen’ recorded the highest germination rate (98.34%) in the M1 medium.Difference of pollens germination rate for five varieties were significant in different culture mediums；with the extension of storage time，the pollen germination rate showed significant decreasing trend in ‘Putaozhi’.The germination rate was from low temperatune germination rate of 87.87% after stored 30 days dropped to 3.18% and 2.02% when the pollen of ‘Putaozhi’ was stored for 50 d and for 70 d，respectively；besides，pollen storage capacity were observed on three varieties which were stored for 70 d with low temperature，and the results showed significant difference for different varieties.Pollen germination rate of ‘Hongluzhen’ was still 38.16%，but the pollen germination rate of ‘Putaozhi’ and ‘Genv’ were only 2.02% and 2.69%.

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Last Update: 2014-08-30