|Table of Contents|

EstablishmentandPrimerScreeningoftheSSR-PCRReactionSystemfor  Armillarialuteo-virens(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年16期
Page:
114-119
Research Field:
Publishing date:

Info

Title:
EstablishmentandPrimerScreeningoftheSSR-PCRReactionSystemfor  Armillarialuteo-virens
Author(s):
LIYing1; XIEZhan-ling 1; 2; TIANFei1; JIAXian-qing1; FANGHui1; XIAMing-zhe1
1.CollegeofEco-EnvironmentalEngineering，QinghaiUniversity，Xining，Qinghai810016；2.NationalKeyLaboratoryBreedingBaseforInnovationandUtilizationofPlateauCropGermplasm，Xining，Qinghai810016
Keywords:
Armillarialuteo-virens; SSR-PCR; systemoptimization; orthogonaldesign; primers
PACS:
S503.53
DOI:
-
Abstract:
TakingDNAextractedfrom Armillarialuteo-virens byCTABmethodastemplate，L16(45)orthogonaldesignwasusedtooptimizethemainfactorsaffectingtheSSR-PCRsystem，whichwereMg2+，dNTPs，primers，Taq DNApolymeraseandtemplateDNAconcentration.ItestablishedtheoptimumSSR-PCRreactionamplificationsystemof Armillarialuteo-virens，andbasedontheoptimumSSR-PCRreactionamplificationsystemannealingtemperaturewasselected.Theresultsshowedthata15μLreactionsystemcontained1×PCRBuffer，100ngtemplateDNA，dNTPs 217μmol/L，primer0.5μmol/L，TaqDNApolymerase0.75U，Mg2+1.3mmol/L.Thereactionsystemwasprovedingoodstabilityandreproducibility.Atthesametime，8primercombinationswereselectedwiththeoptimizedsystemamong34primercombinations，whichhadabundantpolymorphismbands.Thisstudycouldbeusedinanalysisofgeneticdiversity，geneticrelationshipandfingerprintin Armillarialuteo-virens.

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Last Update: 2014-09-12