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StudiesonCallusInductionandTransientExpressionof  Liliumtenuifolium(Oriental×Trumpet)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年16期
Page:
103-107
Research Field:
Publishing date:

Info

Title:
StudiesonCallusInductionandTransientExpressionof  Liliumtenuifolium(Oriental×Trumpet)
Author(s):
QUANYong-hui 1; 2; LIUYa-li 1; 2; QIYin-yan 1; 3; JIANGLing 1; 2; TIANFei-fei 1; 2; DULing-juan 1; 2
1.StateKeyLaboratoryofCropStressBiologyinAridAreas(NorthwestAgriculturalandForestryUniversity)，KeyLaboratoryofHorticulturalPlantBiologyandGermplasmInnovationinNorthwestChina，MinistryofAgriculture，Yangling，Shaanxi712100；
2.CollegeofForestry，NorthwestAgriculturalandForestryUniversity，Yangling，Shaanxi712100；3.CollegeofHorticulture，NorthwestAgriculturalandForestryUniversity，Yangling，Shaanxi712100
Keywords:
lily; callus; plantregeneration; GUS genetransientexpression
PACS:
S682.2+65
DOI:
-
Abstract:
Taking LiliumtenuifoliuOriental×Tumpet‘Robina’asmaterials,andadventitiousbudswhichdifferentiatedfrombulbscales，filamentsandstalkofitwereusedtoformembryogeniccallus,multiplicationwasconductedviasolid,liquidandliquidpreculturethensolidculturemethods.Theresultsshowedthatitproducedthemostembrogeniccalluswhenexplantswereculturedoninductionmediumwhichcontained0.5mg/LNAA.Thebestresultofcallusproliferationwasmade viaaalternativeculturemethodwhichcalluswastransferredonsolidandliquidmediumbyturns.The GUSgenecontainedinvectorpCAMBIA1301wasintroducedintotheembryogenicculturesbyAgrobacterium-mediatedtracientexpressionandthenGUSchemicaltissuesstainingwasconductedtoobserve.Theresultsshowedthatitwasnosignificanttransientexpressionefficiencyvarianceamongcallusofthreecultivationmethods.

References:

[1]NhutDT,LeBV,MinhNT,etal.Somatic embryogenesisthroughpseudo-bulblettransversethincelllayerofLiliumlongiflorum[J].PlantGrowthRegul,2002,37:193-198.
[2]李筱帆.几种百合组织培养及体细胞胚发生技术的研究[D].北京:北京林业大学,2009. 
[3]MercuriA,BenedettiLD,BrunaS,etal.Agrobacterium-mediated transformationwithrolgenesofLiliumlongiflorum Thunb[J].ActaHort,2003,612:129-136.
[4]HoshiY,KondoM,MoriS,etal.ProductionoftransgeniclilyplantsbyAgrobacterium-mediatedtransformation[J].PlantCellRep,2004,22:359-364.
[5]OgakiM,FuruichiY,KurodaK,etal.Importanceofco-cultivationmediumpHforsuccessful.Agrobacterium-mediatedtransformationof Lilium×formolongi[J].PlantCellRep,2008,27:699-705.
[6]张萍.百合转蓝色基因品种的选择及其遗传转化研究[D].杨凌:西北农林科技大学,2011.
[7]石晋芳,孙明,张启翔,等.百合体细胞胚胎发生研究进展[J].北方园艺,2010(18):215-219.
[8]ChinWH,WeiTJ,HuiCL,etal.Plantregenerationviasomaticembryogenesisfromsuspensioncellculturesof Lilium×formolongi usingabioreactorsystem[J].InVitroCellDevBiolPlant，2006,42:240-246. 
[9]HaenschKT.Plantregenerationthroughsomaticembryogenesisin differentgenotypesof Lilium-hybrids[J].Gartenbauwissenschaft,1996,61(5):214-218.
[10]GodoT,KobayashiK.InvitropropagationutilizingsuspensionculturesofmeristematicnodularcellclumpsandchromosomestabilityofLilium×formolongi Hort[J].ScientiaHorticulturae,1998,72:193-202.
[11]NhutDT,MinhHanhNT,TuanPQ,etal.Liquidcultureasapositiveconditiontoinduceandenhancequalityand quantityofsomaticembryogenesisof Liliumlongiflorum[J].Scientia Horticulturae,2006,110:93-97.
[12]王世玉,郑用琏,张方东,等.玉米成熟胚胚性愈伤组织的诱导、高频再生基转化的研究[J].作物学报,2008,34(3):423-428.

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Last Update: 2014-09-12