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Optimization of  Agrobacterium mediated Transformation Systems of  Alfalfa Cotyledonary Node(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年15期
Page:
109-113
Research Field:
Publishing date:

Info

Title:
Optimization of  Agrobacterium mediated Transformation Systems of  Alfalfa Cotyledonary Node
Author(s):
MA Dong-mei1; LI Hui-wen2; GUO Lin-na2; JIN Feng-xia1; XU Xing1; 2
1.College of Agriculture,Ningxia University,Yinchuan,Ningxia 750021；
2.College of Life Science,Ningxia University,Yinchuan,Ningxia 750021
Keywords:
-
PACS:
S 541+1
DOI:
-
Abstract:
Taking Medicago sativa ‘Jin Queen’ as material,taking cotyledonary nodes of alfalfa as transformation receptors,the effects of transformation of alfalfa cotyledonary node of factors,the cotyledon sections physiological period,infection time,the co-culture time on transformation frequency via  Agrobacterium mediated transformation method were investigated.The object was to optimize transformation procedure,and lay a foundation for establishing the efficient alfalfa cotyledon node genetic transformation system.The results showed that infection with Agrobacterium (OD600=0.6),the optimal receptor material was 9-day-old cotyledon node,infection time 20 min,co-culture time 3d.The inhibitory concentration of cefotaxime was 500 mg/L.

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Last Update: 2014-08-12