|Table of Contents|

Study on Systemic Chromatography Separation Methods of Chemical Compounds from Cinnamomi ramulus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年14期
Page:
107-111
Research Field:
Publishing date:

Info

Title:
Study on Systemic Chromatography Separation Methods of Chemical Compounds from Cinnamomi ramulus
Author(s):
WANG Xi-ning1; ZHU Jing-bo1; 2; DING Yan1; 2; KOU Zi-nong2; WANG Zhen-zhong3; XIAO Wei3
1.School of Food Science and Technology,Dalian Polytechnic University,Dalian,Liaoning 116034；2.Institute of Chemistry and Application of Plant Resources,Dalian Polytechnic University,Dalian,Liaoning 116034；
3.Jiangsu Kanion Pharmaceutical Company Limited,Lianyungang,Jiangsu 222001
Keywords:
Cinnamomi ramulus; chromatography separation; thin layer chromatography; preparative chromatography
PACS:
S 503.53
DOI:
-
Abstract:
Taking Cinnamomi ramulus as material,the systemic chromatography separation methods including pre treatment of samples,selection of chromatography separation conditions and the estimate of parameters about preparative chromatography were systemically studied.Elution volumes of reagents and retention volume of each compounds could be calculated and estimated accurately based on the Rf value of TLC data and moving velocity of compounds u=u0/(1+k) in silica gel chromatography which avoided the blindness of separation process.The results showed that extracted by methanol and nhexane extraction were effective methods for the enrichment of main compounds of Cinnamomi ramulus；the systemic TLC methods studied and built would select chromatography packing,reagents systems and reagents proportion quickly and effectively.For main compounds of Cinnamomi ramulus the best chromatography separation packing was silica gel and the best elution system was nhexane/ethyl acetate.8 compounds were separated by once preparative chromatography separation which conditions were selected by this method and purity of 5 fractions were greater than 95% detected by HPLC.Systemic chromatography separation methods for chemical compounds of Cinnamomi ramulus were built.

References:

[1]刘江云.常用中药桂枝化学成分的研究[D].北京:中国协和医科大学,2002.

[2]Liu C,Zhong S M,Chen R Y,et al.Two new compounds frm the dried tender stems of Cinnamomum cassia[J].J Asian Nat Prod Res,2009,11(9):845-849.
[3]Tran M N,Do T H,Ik S L,et al.Two new diterpenes from the twigs of Cinnamomum cassia[J].Helv Chim Acta,2009,92:2058-2062.
[4]Tran M N,Ik S L,Do T H,et al.Tyrosinase inhibitory constituents from the twigs of Cinnamomum cassia[J].J Nat Prod,2009,72:1205-1208.
[5]Sun Y K,Yean K K,Ja Y K,et al.Platelet anti aggregation activities of compounds from Cinnamomum cassia[J].J Med Food,2010,13(5):1069-1074.
[6]Lu D Y,Kang Q S.Theory and practice from TLC to industrial preparative chromatography(IPC)in the separation of natural product[J].Natural product research and development,2004,16:153-156.
[7]Justin D F,Chad M K.Flash column chromatograms estimated from thin ayer chromatography data[J].J Chromatography A,2008,1211:49-54.
[8]Tero W,Irena V,Pia V,et al.Use of drylab for simulation of TLC separation and method transfer from TLC to HPLC[J].J Planar Chromatography,2009,19:118-122.
[9]许琴永.药食两用中药抑制α葡萄糖苷酶活性及作用机制研究[D].大连:大连工业大学,2010.
[10]卢佩章.高效液相色谱法及其专家系统[M].大连:辽宁科学技术出版社,1991:322-359.
[11]Ding Y,Wu E Q,Liang C,et al.Discrimination of cinnamon bark and cinnamon twig sample sourced from various countries using HPLC based fingerprint analysis[J].Food Chemistry,2011,127:755-760.
[12]张艺轩.基于液质联用技术的温络方药效物质基础研究[D].北京:北京协和医学院,2010.
[13]Szabolcs N,Karin D T,Georg C,et al.Strategies of mobile phase transfer from thin layer to medium pressure liquid chromatography with silica as the stationary phase[J].J Chromatography A,1990,499:453-462.

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Last Update: 2014-08-10