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Establishment of Regeneration System in vitro from Cotyledonary Nodes of Medicago sativa L.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年14期
Page:
104-106
Research Field:
Publishing date:

Info

Title:
Establishment of Regeneration System in vitro from Cotyledonary Nodes of Medicago sativa L.
Author(s):
MA Dong-mei1; WANG Jing2; JIN Feng-xia1; GUO Lin-na2; XU Xing1; 2
1.College of Agriculture,Ningxia University,Yinchuan,Ningxia 750021；
2.College of Life Science,Ningxia University,Yinchuan,Ningxia 750021
Keywords:
Medicago sativa L.; cotyledonary node; adventitious shoot; regeneration system
PACS:
S 541+.1
DOI:
-
Abstract:
Taking the cotyledonary nodes of Medicago sativa as explants and MS as the basic medium,the effect of different proportion of hormones on explants differentiation,adventitious bud induction,elongation and rooting were studied.The results showed that the optimum medium for alfalfa cotyledon node regeneration：germination medium was MS；the best induction medium was MS+1.0 mg/L 6BA,with induction time 7 to 10 d；the best adventitious shoot elongation medium was MS+0.5 mg/L 6BA；the best rooting medium was 1/2MS+0.1 mg/L IBA,root could be formed in 15 days.

References:

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Last Update: 2014-08-10