Study on Micropropagation Technique System of Mentha spicata Linn(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Author(s):: SHEN Shun-xian; LI Xue-hui; HE Ai-li; FAN Ya-min; Henan Vocational College of Agricultural，Zhongmu，Henan 451450

Keywords:: Mentha spicata Linn; tissue culture; micropropagation technique; orthogonal test

Abstract:: Using the tender stem containing terminal bud in spring as explants，the orthogonal test was used,the micropropagation technique system of Mentha spicata ?Linn were studied.The results showed that the best method for disinfectant was 70% to 75% alcohol (washed for 30 s) and 01% mercuric chloride (washed for 6 min).The optimal medium for inducing shoot was MS+NAA 0.05 mg/L+6BA 1.5 mg/L+30 g/L sugar+7 g/L agar；the optimal medium for shoot generation was MS+6BA 0.5 mg/L+NAA 0.01 mg/L+GA₃?0.1 mg/L+20 g/L to 30 g/L sugar+7 g/L agar；the optimal medium for rooting was 1/2MS+IBA 0.2 mg/L+KT 0 to 0.05 mg/L+20 g/L to 25 g/L sugar+7 g/L agar.

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Last Update: 2014-08-08