Clustered Buds Proliferation Optimization of Culture Conditions of Dendrobium candidum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Clustered Buds Proliferation Optimization of Culture Conditions of Dendrobium candidum

Author(s):: PAN Mei; WANG Jing-fei; JIANG Dian-qiang; HUANG Sai; LV De-ren; QI Hua-sha; Institute of Garden Flower，Hainan Academy of Agricultural Sciences，Haikou，Hainan 571100

Keywords:: Dendrobium candidum; clustered buds; proliferation culture; optimization

Abstract:: In order to select optimal medium and culture conditions from clustered buds subculture proliferation of Dendrobium candidum，taking the MS as the basic medium,the effect of activated carbon，plant hormones，organic additives，temperature，light and other factors on growth of Dendrobium candidum were studied.The results showed that the best formola for clustered buds subculture proliferation was MS+6BA 2.0 mg/L+NAA 0.2 mg/L+banana 100 g/L+activated carbon 0.5 g/L+sucrose 30 g/L，proliferation coefficient reached 7.16 in 60 days;optimal temperature was 25℃，with optimal light intensity for 2 000 lx.

[1]林丛发,钟爱清,林云斌，等.铁皮石斛类原球茎增殖和分化的研究[J].江西农业学报,2007,19(1):84-86.

[2]莫昭展,贝学军,韦江萍，等.不同培养条件对铁皮石斛原球茎增殖的影响[J].安徽农业科学,2007,35(22):6835-6836,7036.

[3]洪香娇,瑜晚之,熊正葵.铁皮石斛的组织培养与快速繁殖[J].现代园艺,2008(7):9-10,13.

[4]张书萍,白石,陈丽静.铁皮石斛的组织培养与快速繁殖[J].辽宁农业科学,2008(6):12-15.

[5]李进进.铁皮石斛茎段离体初代培养研究[J].作物杂志,2010(1):79-80.

[6]杨立昌,乙引,张宇斌.铁皮石斛快速繁殖体系研究[J].北方园艺,2010(2):136-138.

[7]戴小英,张淑霞,周莉莉，等.铁皮石斛不同外植体组培快繁技术比较研究[J].中国农学通报,2011,27(10):122-126.

[8]李莹,谭鹏鹏,彭方仁，等.铁皮石斛组培快繁技术[J].林业科技开发,2012,26(1):96-99.?

[9]王玉英,高新一.植物组织培养技术手册[M].北京:金盾出版社,2006:46.

Last Update: 2014-08-08