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Effect of Different Culture Condition on Embryogenic Callus in Vitro of Citrus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年13期
Page:
125-127
Research Field:
Publishing date:

Info

Title:
Effect of Different Culture Condition on Embryogenic Callus in Vitro of Citrus
Author(s):
LUO Jun-qin; XU Jian-guo; WANG Ping
Zhejiang Citrus Research Institute，Huangyan，Zhejiang 318020
Keywords:
citrus; callus; induction; influence factors
PACS:
S 6661
DOI:
-
Abstract:
Taking 5 different varieties of citrus as materials，the effect of different hormone concentrations，explants and varieties on embryogenic callus induction of citrus in vitro were studied.The results showed that the optimal hormone concentration media for inducing embryogenic callus from hypocotyls was MT+BA 1.0 mg/L+NAA 0.5 mg/L，while that for cotyledons was MT+BA 2.0 mg/L+NAA 1.0 mg/L or much higher;the ability of forming embryogenic callus from explants were hypocotyls>cotyledons，rootlets>epicotyls;and the ability of induced embryogenic callus from hypocotyls for citrus varieties were ‘Bendizao mandarin’>‘Calamondin’，‘Nanfeng mandarin’>‘Luiben orange’>‘Oval kumquat’.

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Last Update: 2014-08-08