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Analysis of the Correlation Between Baicalin and Peroxidase Activity in the Callus of Scutellaria baicalensi Georgi(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年13期
Page:
108-111
Research Field:
Publishing date:

Info

Title:
Analysis of the Correlation Between Baicalin and Peroxidase Activity in the Callus of Scutellaria baicalensi Georgi
Author(s):
JIAO Zhan-zhan; ZHANG Dong-xiang; ZHAO Jing; JIA Bin; MENG Qing-wei; ZHANG Chao
College of Life Science and Forestry，Qiqihar University，Qiqihar，Heilongjiang 161006
Keywords:
Scutellaria baicalensis Georgi; callus; baicalin; peroxidase activity; orthogonal design
PACS:
S 503.53
DOI:
-
Abstract:
Taking the callus of Scutellaria baicalensis Georgi as material，the relationship between the variation of peroxidase activity and baicalin secondary synthesis in the growth process of the callus of Scutellaria baicalensis Georgi were studied.The results showed that during the growth cycle (27 days) of the callus of Scutellaria baicalensis Georgi，the activity of the level of peroxidase and the accumulation of baicalin was positively correlated.During the former 15 days of the growth cycle of the callus of Scutellaria baicalensis Georgi，the skullcap callus was in its growth phase.The activity of the peroxidase express at a low level.The synthesis of baicalin was slow.During 15 to 21 days，the callus was in its stable phase.The activity of peroxidase express at high levels and reached its maximum，at the same time baicalin rapidly synthetised and reached its maximum.During 21 to 27 days，the callus was in its aging period，while the activity of peroxidase reduced.The synthesis of baicalin almost stopped.L9?(34) orthogonal table was used to optimize the ratio of medium 6BA，carbon sources，nitrogen sources，the pH.The optimal culture conditions were 6BA 2 mg/L，carbon source：20 g sucrose+10 g glucose，nitrogen source (NH4+) 30 mmol/L，pH 6-0.

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Last Update: 2014-08-08