|Table of Contents|

Research Progress of Plant Virus Eradication Using Super Low Temperature Processing(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年12期
Page:
184-187
Research Field:
Publishing date:

Info

Title:
Research Progress of Plant Virus Eradication Using Super Low Temperature Processing
Author(s):
YANG Zhi1; 2; CHEN Chun-ling1; 3; XU Mei-long1; 3
1.The National Center of Research and Engineering Technology of Economic Forest Tree Speedy Propagation，Yinchuan，Ningxia 750004;
2.College of Life Science，Ningxia University，Yinchuan，Ningxia 750021;
3.State Key Laboratory of Seeding Bio-engineering，Yinchuan，Ningxia 750004
Keywords:
super low temperature processing; peel tubes; heat treatment; plant virus; virus eradication
PACS:
TB 66;Q 813.1+2
DOI:
-
Abstract:
Super low temperature processing is an emerging plant virus eradication technology based on cryopreservation and plant tissue culture.This method is independent of the size of shoot tips used with advantages of easy manipulation，short experimental period and high efficiency of pathogen eradication.The principle，advantages，disadvantages and steps of super low temperature processing were summarized in order to improve its understanding，and its application prospects were briefly discussed.

References:

[1]朱述钧,王春梅,陈浩.抗植物病毒天然化合物研究进展[J].江苏农业学报，2006,22(1):86-90.
[2]陈芳,王子成.植物脱毒新技术低温疗法[J].河南农业科学，2006(12):11-13,81.
[3]曹雪松.植物病毒在细胞内和细胞间运动的研究[J].莱阳农学院学报，2002,19(4):251-252.
[4]张振臣,李大伟,于嘉林,等.植物病毒细胞间运动及运动蛋白基因介导的抗病性研究进展[J].农业生物技术学报，2000,8(4):403-408.
[5]洪健,陈集双,周雪平,等.植物病毒的电镜诊断[J].电子显微学报,1999,18(3):274-289.
[6]Wei T，Zhang C，Hong J，et al.Formation of complexes at plasmodesmata for potyvirus inercellular movement is mediated by the viral protein P3N-PIPO[EB/OL].2010，6(6):e1000962[http://www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1000962].
[7]陈齐斌,沈嘉祥.抗植物病毒剂研究进展和面临的挑战与机遇[J].云南农业大学学报,2005,20(4):505-512. [8]Brison M，de Boucaud M T，Pierronnet A，et al. Effect of cryopreservation on the sanitary state of a cv.Prunus rootstock experimentally contaminated with plum pox potyvirus[J].Plant Science,1997,123:189-196.
[9]Helliot B，Panis B，Poumay Y,et al. Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.) [J].Plant Cell Reports，2002,20:1117-1122.
[10]Wang Q C，Valkonen J P T.Efficient elimination of sweetpotato little leaf phytoplasma from sweetpotato by cryotherapy of shoot tips[J].Plant Pathology，2008,57:338-347.
[11]Wang Q C，Valkonen J P T.Elimination of two viruses which interact synergistically from sweetpotato by shoot tip culture and cryotherapy[J].Journal of Virological Methods，2008,154:135-145.
[12]Wang Q C,Wilmer J C，Minna-Liisa R,et al.Combined thermotherapy and cryotherapy for efficient virus eradication:relation of virus distribution，subcellular changes，cell survival and viral RNA degradation in shoot tips[J].Molecular Plant Pathology，2008,9(2):237-250.
[13]Wang Q C，Liu Y，Xie Y H，et al.Cryotherapy of potato shoot tips for efficient elimination of potato leafroll virus (PLRV) and Potato virus Y (PVY) [J].Potato Research，2006,49:119-129.
[14]Wang Q C,Munir Mawassi,Li P，et al.Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L[J].Plant Science，2003,165:321-327.
[15]Wang Q C，Jari P T V.Cryotherapy of shoot tips:novel pathogen eradication method[J].Trends in Plant Science，2009,14(3):119-122.
[16]蔡斌华,张计育,渠慎春,等.通过玻璃化超低温处理脱除草莓轻型黄边病毒(SMYEV)研究[J].果树学报，2008,25(6):872-876.
[17]曲先.低温疗法脱除马铃薯病毒及遗传变异分析[D].郑州：河南大学，2009.
[18]Li W B，Hartung,J S，Levy L.Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing[J].Journal of Microbiological Methods，2006,66:104-115.
[19]孙琦,张春庆.植物脱毒与检测研究进展[J].山东农业大学学报(自然科学版)，2003,34(2):307-310.
[20]Wang Q C，Panis B，Engelmann F,et al.Cryotherapy of shoot tips:a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation[J].Annals of Applied Biology，2009,154:351-363.
[21]徐刚标,陈良昌.植物种质超低温保存[J].经济林研究,1998,16(2):15-16,53.
[22]Faccioli G，Marani F.Virus elimination by meristem tip culture and tip micrografting[M].In:Hadidi A，Khetarpal RK，Koganezawa H (eds) Control of plant virus diseases.St.Paul;APS Press,1998:346-380.[23]高尚士.花卉病毒病的检疫及消除方法[J].植物检疫，1994(6):340-341.
[24]王永伟,王慧霞,贺丹,等.观赏植物病毒病害及病毒脱除研究现状与发展趋势[J].中国农学通报，2008,24(5):313-317.
[25]Ding F,Jin S X,Hong N，et al.Vitrification-cryopreservation，an efficient method for eliminating Candidatus Liberobacter asiaticus，the citrus Huanglongbing pathogen，from in vitro adult shoot tips[J].Plant Cell Reports，2008,27:241-250.
[26]梁宏,王起华.植物种质的玻璃化超低温保存[J].细胞生物学杂志，2005,27(1):43-45.

Memo

Memo:
-
Last Update: 2014-08-20