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Extraction and Elimination of Virus of dsRNA from Pleurotus ostreatus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年12期
Page:
111-113
Research Field:
Publishing date:

Info

Title:
Extraction and Elimination of Virus of dsRNA from Pleurotus ostreatus
Author(s):
FU Yue-yue; YANG Hong-yi
College of Life Science，Northeast Forestry University，Harbin，Heilongjiang 150040
Keywords:
Pleurotus ostreatus; dsRNA; elimination of virus
PACS:
S 646.1+4
DOI:
-
Abstract:
Taking Pleurotus ostreatus as material，the special dsRNAs of virus were extracted from Pleurotus ostreatus with modified SDS method，and elimination of virus in Pleurotus ostreatus was performed with reagent-mycelial tip-cutting and high temperature-mycelial tip-cutting methods.The results showed that the dsRNA was the same as one in foreign studies.The virus could be effectively eliminated with reagent-mycelial  tip-cutting method，and the methods，including of ribavirin-mycelial tip-cutting，cycloheximide-mycelial tip-cutting，ribavirin and moroxydine hydrochloride-mycelial tip-cutting，could reducethe quantity of dsRNA of virus.The system of extraction of dsRNA was developed using tissues of Pleurotus ostreatus as material.In addition，the technique could effectively detect virus in Pleurotus ostreatus.

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Last Update: 2014-08-19