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Study on the Callus Induction from Epimedium koreanum Nakai(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年12期
Page:
106-108
Research Field:
Publishing date:

Info

Title:
Study on the Callus Induction from Epimedium koreanum Nakai
Author(s):
WANG JingLI JingJIA Ling-yunZENG Wen-wenLIU Peng-pengLU Jin-cai
School of Traditional Chinese Medicine,Shenyang Pharmaceutical University,Shenyang,Liaoning 110016
Keywords:
Epimedium koreanum Nakaicallusinduction
PACS:
S 503.53
DOI:
-
Abstract:
Taking the young leaf,stem apex and petiolesuitable of Epimedium koreanum Nakai as explants,the condition of its callus induction was studied,in order to provide scientific basis for large scale production of tissue culture.The results showed that the stem apex was the best production material of tissue culture.The optimum medium for callus induction was MS+2,4-D 2.0 mg/L+6-BA 0.5 mg/L+NAA 1.0 mg/L.In the temperature of 25℃,the induction effect was good under the cultured conditions of dark,and taken out,then cultured in the light intensity 2 000 lx.

References:

[1]《中华人民共和国药典》2010版[S].1部.2010:306-308.
[2]张著林,储蓉,孙超.粗毛淫羊藿种子繁殖试验初报[J].种子,2007,26(3):102-103.
[3]郭玉蓉,张华峰,杨晓华,等.药用植物淫羊藿资源可持续利用现状与展望[J].植物学报,2009,44(3):363-370. [4]罗莉,李燕,杨瑞武,等.柔毛淫羊藿愈伤组织诱导及其总黄酮含量测定[C].第九届全国药用植物及植物药学术研讨会,2010.
[5]盛茂银,杨政富,林菊,等.粗毛淫羊藿幼叶愈伤组织的生产[J].种子,2005,24(9):22-24.
[6]韩素菊,黎云祥,胥晓,等.箭叶淫羊藿胚性愈伤组织诱导及其黄酮类化合物的总含量测定[J].中国中药杂志,2007,32(23):2551-2553.

Memo

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Last Update: 2014-08-19