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Study on the Rapid Propagation of Rosa ‘St.Stwithum’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年12期
Page:
97-99
Research Field:
Publishing date:

Info

Title:
Study on the Rapid Propagation of Rosa ‘St.Stwithum’
Author(s):
XU Ling-ling1; DANG Yue-fang1; TAO Gui-rong1; PEI Xin2; GUO Bin3
1.School of Biotechnology，Xi’an University of Arts and Science，Xi’an，Shaanxi 710065;
2.Xi’an Fangtian Rose Agricultural Science and Technology Co.，Ltd.，Xi’an，Shaanxi 710061;
3.Key Laboratory of Resource Biology and Biotechnology in Western China，School of Life Science，Northwest University，Xi’an，Shaanxi 710069
Keywords:
Rosa ‘ St.Stwithum’; tissue culture; adventitious bud; NAA; IBA
PACS:
S 685.12
DOI:
-
Abstract:
Taking the axillary buds of Rosa ‘St.Stwithum’ as explants，an efficient micropropagation system for Rosa ‘St.Stwithum’ were studied.The results showed that 95.7% adventitious buds induction frequency and 8.4 buds per explant were achieved when cultured on MS media containing 0.05 mg/L NAA and 3 mg/L 6-BA.After transferred the adventitious buds，which length had grown to 3 cm，to rooting media，root organogenesis occurred.The control without any exogenous auxins treatment，which average roots number per stem，induction frequency and the average roots’ length were 6.3 roots，92% and 3.7 cm，respectively，had no significant differences with 0.2 mg/L IBA treatments.High concentrations of NAA and IBA treatments induced a lot of callus，and significantly decreased adventitious roots.92% of the regenerated plantlets survived and grew vigorously in greenhouse condition.

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Last Update: 2014-08-19