|Table of Contents|

Study on the Factors Affecting the Agrobacterium Mediated Transformation of  Brassica oleracea var.botrytis(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年12期
Page:
93-96
Research Field:
Publishing date:

Info

Title:
Study on the Factors Affecting the Agrobacterium Mediated Transformation of  Brassica oleracea var.botrytis
Author(s):
YU Ya; DUAN Wei
Department of Bioengineering，Wuhan Bioengineering Institute，Wuhan,Hubei 430415
Keywords:
broccoli; transformation; PCR; GUS
PACS:
S 635.3
DOI:
-
Abstract:
Taking Brassica oleracea var.botrytis variety ‘Lvxiu’ as material，cotyledons with petiole as explants，Agrobacterium tumefaciens strain LBA4404 were used to transformation system，and the factors affecting the Agrobacterium mediated transformation of Brassica oleracea var.botrytis were studied.The results showed that the concentration of acetosyringone，OD600value，infection time had certain effect on transient expression.Among which OD600value had the most influence，followed by the concentration of acetosyringone and infection time.The optimum condition was 100 μmol/L acetosyringone，OD600value 0.3，infection time 5 min.The resistant plants demonstrated by PCR，Southern hybrization analysis and GUS staining showed that the target gene had been integrated into the broccoli genome and had been well expressed.

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Last Update: 2014-08-19