|Table of Contents|

RAPD Analysis on the Genetic Diversity of Genus Rabdosia in Northeast China(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年12期
Page:
87-90
Research Field:
Publishing date:

Info

Title:
RAPD Analysis on the Genetic Diversity of Genus Rabdosia in Northeast China
Author(s):
ZHANG Bo; LIU Cui-jing; LIU Xia
College of Chinese Medicinal Materials，Jilin Agricultural University，Changchun，Jilin 130118
Keywords:
Rabdosia japonica var.glaucocalyx; Rabdosia excise; RAPD; cluster analysis
PACS:
Q 949.95
DOI:
-
Abstract:
Taking 24 samples of Rabdosia japonica (Burm.f.) Hara var.glaucocalyx (Maxim.) Hara and Rabdosia excise (Maxim.) Hara from different regions of northeast China as materials，the genetic diversity was studied using random amplified polymorphism DNA (RAPD) markers.The results showed that 5 primers were selected from 60 primers.63 DNA bands were amplified from the two species of Rabdosia，the percentage of polymorphism was 87.30%.Analysis of Nei’s unbiased genetic distance showed that the genetic relationship between the two species of Rabdosia was significant.The genetic diversity was high，and the genetic diversity of interspecies was higher than that of interspecies.In conclusion,RAPD molecular marker technology could be an efficient method to study the genetic diversity of Rabdosia plants.

References:

[1]孙骏,郭莉.我国药用香茶菜属植物化学及药理学研究新进展[J].中草药,2002,33(8):3.
[2]那微,鲍建材.中药尾叶香茶菜研究现状及药用前景展望[J].人参研究,2006(1):13-17.
[3]曹露哗,陈子君.蓝萼香茶菜研究进展[J].云南中医中药杂志,2004,25(3):42-43.
[4]王勤,周至品,李爱媛.香茶菜属植物药理活性研究进展[J].现代医药卫生,2008,24(3):362-363.
[5]沈晓丹,王冰.蓝萼香茶菜的化学成分研究[J].中草药,2009,40(12):1883-1885.
[6]王福东,丁兰.蓝萼香茶菜三萜成分的研究[J].中国中药杂志,2005,30(24):1929-1932.
[7]海广范,李生莹.蓝萼香茶菜乙素对AGZY细胞周期及凋亡的影响[J].中国病理生理杂志,2010,26(7):1420-1422.
[8]张崇禧,王雪.长白山地区尾叶香茶菜化学成分的研究[J].中成药,2008,30(1):102-105.
[9]吴月霞,张伟.尾叶香茶菜化学成分的研究[J].中草药,2011,42(12):2402-2406.
[10]Ma Y H，Dang R L，Wu X，et al.RAPD Analysis of genetic relationships in Ephdera in termedia from three local populations in sinkiang[J].Chin Pharm J,2003,38(6):414-415.
[11]Li X，Shao A J，Huang L Q，et al.Study on genetic differentiations among different strains of cultivated ginseng[J].Chin Pharm J,2005,40(15):1135-1137.
[12]Ma X J，Wang X Q，Xu Z X，et al.RAPD Variation within and among populations of Ginseng cultivars[J].Acta Bot Sin,2000,42:587-590.
[13]Schierwater B，Ender A.Different themostable DNA polymerase may amplify different RAPD productcs[J].Nucl Acids Res,1993,21:4647-4648.
[14]Skroch P，Nienhuis J.Impact of scoring error and reproducibility of RAPD data on RAPD based estimates of genetic distances[J].Theor Appl Genet,1995,91:1086-1091.

Memo

Memo:
-
Last Update: 2014-08-19