|Table of Contents|

Detection of the Laccase and MCMase Activities of Agrocybe cylindracea During Its Growth(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年11期
Page:
149-151
Research Field:
Publishing date:

Info

Title:
Detection of the Laccase and MCMase Activities of Agrocybe cylindracea During Its Growth
Author(s):
JIANG Xing-jian; WANG Chun-hui; PENG Yun-xiang; HU Ru-xiao; SHI Zhi-jun; YIN Yong-gang
Institute of Hunan Edible Fungi，Changsha，Hunan 410013
Keywords:
Agrocybe cylindracea; laccase; CMCase; enzyme activity
PACS:
S 646.1+9
DOI:
-
Abstract:
Aim to analysis the degradation ability of mycelia to cellulose，laccase and CMCase activitites from Agrocybe cylindracea were tested in the liquid and fruiting body cultured periods by using ABTS method and DNS method，respectively.The results showed that in the liquid period the laccase activity and CMCase activity were in a low level in the first 6 and 4 days.Then the activities were hastily rised，it was highest up to the 8th and the 6th days and then it was hastily declined until to a low and relaxative level，respectively.In the fruiting body cultured period，the laccase activity and CMCase activity were in a low and common level when the mycelia grow to a half cultural bag，respectively.The laccase activity was a higher level in the first time of primordium formation，and the highest level in the first time of young fruiting body formation.However，the CMCase activity was in a common level before the mycelia grow full of the cultural bag，then up to the highest level in the first time of primordium formation，and then hastily declined to a common level.The laccase activity and CMCase activity were both in a low level in the second fruiting body period.This study provided a reference for the analysis of physiological activity and culture production and utilization of A.cylindracea.

References:

[1]路等学,王龙,张铎,等.茶薪菇胞外酶学性质研究[J].中国酿造,2010(2):37-39.
[2]才晓玲,安福全,邹瑶,等.柱状田头菇研究进展[J].食用菌学报,2011,18(2):65-69.
[3]Uhart M,Piscera J M,Albertó E.Utilization of new naturally occurring strains and supplementation to improve the biological efficiency of the edible mushroom  Agrocybe cylindrace a[J].Ournal of Industrial Microbiology and Biotechnology,2008,35:595-602.
[4]赵爽,刘宇,许峰,等.姬松茸4个品种菌丝体的生物酶活性测定[J].中国食用菌,2010,29(3):32-33.
[5]姜性坚,王春晖,彭运祥,等.5个姬菇菌株不同生长阶段漆酶及纤维素酶活力测定[J].食用菌,2010(6):9-10. [6]黄年来,林志彬,陈国良.中国食药用菌学[M].上海:上海科学技术文献出版社,2010:607-619.
[7]初洋,倪新江,杨佳文,等.姬菇和鲍鱼菇生长期间8种胞外酶活性变化比较[J].烟台大学学报(自然科学与工程版)，2008,21(2):138-142.
[8]郭倩,何庆邦.四孢蘑菇生长过程中四种胞外酶活性和木质纤维素降解的变化规律[J].食用菌学报,1998,5(2):13-17.
[9]Claydon N,Allar M,Wood D A.Fruitbody biomass regulated production of extracellular endocellulase during periodic fruiting by Agaricus bisporus[J].Trans Br Mycol Sci,1988,90(1)：85-90.
[10]暴增海,马桂珍,吴智艳.鸡腿蘑对基质的降解及有关酶活性变化特点研究[J].北方园艺,2002(3):54-55.

Memo

Memo:
-
Last Update: 2014-08-19