|Table of Contents|

Study on Identification of Tomato Biotyoe Fusarium oxysporum f.sp.lycopersici(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年11期
Page:
100-103
Research Field:
Publishing date:

Info

Title:
Study on Identification of Tomato Biotyoe Fusarium oxysporum f.sp.lycopersici
Author(s):
WANG Xiao-xi; GAO Zeng-gui; ZHANG Shuo; ZHOU Yan-bo; WANG Min; KONG Ting-ting
Institute of Plant Immunology,Shenyang Agricultural University,Shenyang,Liaoning 110866
Keywords:
usarium oxysporum; tomato fusarium wilt; rDNA-ITS; cross inoculation
PACS:
S 436.412.1+9
DOI:
-
Abstract:
Taking 27 Fusarium strains as materials,F.oxysporum f.sp.lycopersici was identified using morphology observation and rDNA-ITS sequencing.The results showed that after selected by morphology and rDNA-ITS sequencing,10 F.oxysporum strains were identified by traditional method of specialized form as cross inoculation.Thus N0.YH070001 and DL070009 were confirmed F.oxysporum f.sp.lycopersici. Compared with single traditional method,rDNA-ITS sequencing has higher sensitivity.To test the accuracy and feasibility of this identification method,morphology observation,rDNA-ITS sequencing and traditional method of specialized form identification were used in this study.

References:

[1]徐艳辉,李烨,许向阳.番茄枯萎病的研究进展[J].东北农业大学学报,2008,39(11):128-134.
[2]Gabe H L.Standardization of nomendature for pathogenic races of Fusarium oxysporum f.sp.lycopersici[J].Trans Br Mycol Soc,1975(64):156-159.
[3]Conway W S,Mac Hardy W E.Distribution and growth of Fusarium oxysporum f.sp.lycopersici race l or race 2 within tomato plants resistant or susceptible to wilt[J].Phytopathology,1978,68:938-942.
[4]Gerdemann J W,Finley A M.The pathogenicity of race 1 and 2 of Fusarium oxysporm f.sp.lycopersici[J].Phytopathology,1951,41:238-244.
[5]程爱昀.大棚番茄枯萎病的诊断和防治新技术[J].中国瓜类,2010(1):45-46.
[6]Fravel D,Olivain C,Alabouvette C.Fusarium oxysporum and its biocontrol[J].New phytologist,2003,157:493-502.
[7]张向民.镰刀菌属分类学研究历史与现状[J].菌物研究,2005(2):59-62.
[8]Suga H,Hasegawa T,Mitsui H,et al.Phylogenetic analysis of the phytopathogenic fungus Fusarium solani based on the rDNA-ITS region[J].Mycologica Research,2000,104(10):1175-1183.
[9]Andersen B,Thrane U.Differentiation of Alternaria infectoria and Alternaria alternate based on morphology,metabolitc profiles,and cultural charateristics[J].Can J Microbiol,1996,42:685-689.
[10]Adachi Y,Wantanabe K.Nuclear risbiosomal DNA as a probe for genetic vriability in Japanese pear pathotype Alternaria alternate[M].Appl Environm Microbiol,1993:3197-3205.
[11]Booth.The Genus Fusarium[M].UK(Kew) and USA(Surrey)：Commonwealth Mycological Institute,1971.
[12]Leslie J F,Summerell B A.The Fusarium Laboratory Manual[M].USA：Blackwell Publishing,2006. [13]王拱辰,郑重,叶琪明,等.常见镰刀菌鉴定指南[M].北京:中国农业科技出版社,1996.
[14]Iwen P C,Hinrichs S H,Rupp M E.Utilization of the internal transcribed spacer regions as molecular targets to detect and identify human fungal pathogens [J].Medical Mycology,2002,40:87-109.
[15]Doohan F M,Parry D W,Jenkinson P,et al.The use of species-specific PCR based assays to analyse Fusariumear blight of wheat[J].Plant pathology,1998,47:197-205.
[16]Theresa L,Woongoh D,Seonkim H,et al.Identification of Deoxyni-valenol and Nivalenol-producing chemo types of Cibherella Zeaeby using PCR Applied and Environmental[J].Microbiology,2001,45:2966-2972.
[17]Kulik  T,Fordonski G,Pszczolkowska A,et al.Development of PCR assay based on ITS2 rDNA polymorphism for the detection and differentiation of  Fusarium sporotrichioides[J].Micro biology,2004,239(1):181-186.

Memo

Memo:
-
Last Update: 2014-08-18