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Genetic Diversity Analysis of Twenty-six Species in Rose Class Based on ITS Sequences(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年11期
Page:
94-96
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis of Twenty-six Species in Rose Class Based on ITS Sequences
Author(s):
OU Li-jun; SUN Hai-jun
Key Laboratory of Hunan Higher Education for Hunan-western Medicinal Plant and Ethnobotany,Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources,Department of Life Science,Huaihua University,Huaihua,Hunan 418008
Keywords:
family; ITS sequences; identify; phylogenetic relationship
PACS:
S 336
DOI:
-
Abstract:
The genetic analysis of 26 species including Campanulaceae,Lamiaceae,Asteraceae,Leguminosae sp.and Rosaceae were studied using ITS sequences.The results showed that the ITS lengths of 26 species were from 580 bp to 815 bp and GC content was about 60%.Clustering results based ITS sequences showed the species from the same family were firstly clustered.Five families were divided into two branches that Campanulaceae,Lamiaceae and Asteraceae were a branch and the others were a branch.The results indicated different species from different families could be identified and the identification of familial relationships had some limitations by ITS sequences.In conclusion,ITS sequences would be combined with some analytical tools such as morphology,structure and others molecular techniques to accurately analyze familial relationships.

References:

[1]Takhtajan A.Diversity and classification of flowering plants[M].New York：Columbia University Press,1997.
[2]Thorne R F.Classification and geography of the flowering plants[J].Botanical Review,1992,58:225-348.[3]Dahlgren G.An updated angiosperm classification[J].Botanical Journal of the Linnean Society,1989,100:197-203.
[4]Cronquist A.The evolution and classification of flowering plants[M].New York：The New York Botanical Garden,1988.
[5]Melchior H A.Engler’s syllabus der pflanzenfamilien,zwolfte zuflage,II band,Angiospermen[M].Berlin：Gebruder Bomtrager,1964.
[6]吴征镒,汤彦承,路安民,等.试论木兰植物门的一级分类-一个被子植物八纲系统的新方案[J].植物分类学报,1998,36(5):385-402.
[7]葛颂,Schaal B A,洪德元,等.用核糖体DNA的ITS序列探讨裂叶沙参的系统位置—兼论ITS片段在沙参属系统学研中的价值[J].植物分类学报,1997,35(5):385-395.
[8]施苏华,杜雅青,David B,等.用cpDNA matK基因和nrDNA ITS区序列确定我国特有植物四棱草属的系统位置[J].科学通报,2003,48(11):1176-1180.
[9]杨中保,刘春霞,赵卫敬,等.灯盏花rDNA ITS序列测定及其在替代资源寻找中的意义[J].时珍国医国药,2010,21(10):2629-2632.
[10]高静.基于5.8S rDNA ITS序列的内蒙古棘豆属植物分子系统学研究[D].呼和浩特:内蒙古师范大学,2011. [11]White T J,Bruns T,Lee S,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M].In:Innes M,Gelfand D,Sninsky,etaleds.PCR protocols：a guide to methods and applications,1990:315-322.
[12]郑小艳,曹家树,滕元文.DNA序列分析在植物分子系统学研究中的应用现状-以蔷薇科为例[J].园艺学报,2009,36(12):1827-1836.

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Last Update: 2014-08-18