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Study on Tissue Culture and Rapid Propagation of Lilium dauricum and Lilium cernum(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年11期
Page:
91-94
Research Field:
Publishing date:

Info

Title:
Study on Tissue Culture and Rapid Propagation of Lilium dauricum and Lilium cernum
Author(s):
WANG Na1; GUO Tai-jun1; LI Xue1; CHEN Shao-peng2
1.College of Horticulture,Jilin Agricultural University,Changchun,Jilin 130118；
2.Jilin Academy of Forestry Sciences,Jilin,Jilin 132013
Keywords:
Lilium dauricum; Lilium cernum; tissue culture
PACS:
S 682.2+65
DOI:
-
Abstract:
Taking different parts of squama of the bulb scales of Lilium dauricum and L.cernum as explants,the effect of different sterilize method and different hormones composing and different parts of a squama on their adventitious bud induction and multiplication were studied.The results showed the bulb scales treated with 75% alcohol for 30 s followed by 30% 84 disinfectant for 15 min exhibited the best sterilization effect；the suitable medium for bulb induction of L.dauricum was MS+6-BA 0.5 mg/L+NAA 0.5 mg/L；the optimum medium for adventitious bud proliferation was MS+6-BA 0.3 mg/L+NAA 0.2 mg/L.The suitable medium for bulb induction of L.cernum was MS+6-BA 1.0 mg/L+NAA 0.3 mg/L,MS+6-BA 1.0 mg/L+2,4-D 0.1 mg/L was better for adventitious bud proliferation.The better sampling sites of explants were the base of the bulbs central scales.

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Last Update: 2014-08-18