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SSR Analysis of Genetic Diversity and Fingerprint Database Construction of Grape Germplasm(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年11期
Page:
87-90
Research Field:
Publishing date:

Info

Title:
SSR Analysis of Genetic Diversity and Fingerprint Database Construction of Grape Germplasm
Author(s):
XIAN Li-jie; LIU Xing-ju; LI Xue-yan; LIANG Hai-yong; YANG Min-sheng
Key Lab of Genetic Resources of Forest and Forest Protection of Hebei Province,Hebei Agricultural University,Baoding,Hebei 071000
Keywords:
grape; SSR; genetic diversity; fingerprint database
PACS:
S 663.1
DOI:
-
Abstract:
Taking 45 grape varieties collected from Zhengzhou Fruit Research Institute as materials,the genetic analysis of them were analyzed using SSR markers technology.The results showed that 19 basic core primers for PCR amplification of SSR loci were located on different chromosomes,76 bands were amplified,including 74 polymorphic bands.No.of allelic loci detected by each pair of primer ranged from 2 to 5,with the polymorphic rate of 66.7%~100%.Through the cluster analysis showed that 45 grape materials could be divided into 3 groups in the genetic distance of 0.41.Detection of different primers amplified products of the grape varieties tested,the genotype maps of each species in a set of SSR loci in different chromosomes were gotten.Grape varieties SSR genotype fingerprint library were preliminarily established,which provided a basic data for identification of grape varieties or lines using SSR markers.

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Last Update: 2014-08-18