Study on the Optimal of Separation,Purification and Fusion Conditions of Cucumber Seedlings Cotyledons Protoplast(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:

2013年11期

Page:

6-9

Research Field:

Publishing date:

Info

Title:

Study on the Optimal of Separation,Purification and Fusion Conditions of Cucumber Seedlings Cotyledons Protoplast

Author(s):

DENG Hong-mei; MA Chao; WANG Ying

College of Chemistry and Life Sciences,Guangdong University of Petrochemical Industry,Maoming,Guangdong 525000

Keywords:

cucumber cotyledons; protoplast; separation; purification; fusion

PACS:

S 642.2

DOI:

-

Abstract:

Taking cucumber seedlings cotyledons as materials,the effect of different enzyme concentration,osmotic agent concentration,enzyme solution to time on protoplast yield and activity with orthogonal experiment were studied;and different concentration of sucrose protoplast purification and different PEG concentration on protoplast fusion rate were discussed.The results showed that cucumber seedlings in cotyledons containing 1.0% cellulose enzyme,0.8% pectinase and 0.3 mol/L of mannitol in liquid enzymes mix enzyme solution 3 hours,would obtain the highest vibrant protoplast,about 7.2×10.6pieces/g,protoplast vigor for 83.72%.When sucrose density was 20% the purified effect was the best,it could get vigorous protoplast yield 7.1×10.6pieces/g；PEG concentration of 40% had the highest rate in the fusion,fusion rate of 32%.

References:

[1]高东迎,黄雪清,孙立华.水稻体细胞杂交研究进展[J].生物工程进展,2001,21(3):38-42.

Memo

Memo:

-

Common functions