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Development of Liquid Chip Technique for Rapid Detection of Tomato Black Ring Virus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年01期
Page:
127-130
Research Field:
Publishing date:

Info

Title:
Development of Liquid Chip Technique for Rapid Detection of Tomato Black Ring Virus
Author(s):
ZHANG Yong-jiang1; XIN Yan-yan1; LI Gui-fen1; LIU Zhong-mei2; LIU Hong-yi2
1.Chinese Academy of Inspection and Quarantine，Beijing 100029；
2.Technical Center of Inspection and Quarantine，Heilongjiang Entry-Exit Inspection and Quarantine Bureau，Harbin，Heilongjiang 150001
Keywords:
Tomato black ring virus(TBRV); liquid chip technique; detection
PACS:
S 436.412.1+1
DOI:
-
Abstract:
To develop liquid chip technique for detecting Tomato black ring virus (TBRV)，the nucleotide sequence of TBRV was analyzed by using the software Primer Premier 5.0，and specific probe labeled with biotin was prepared and coupled with fluorescence-coded microspheres，which was used for hybridization reaction to RT-PCR products of TBRV.Liquid chip detection method for TBRV was established by using Bio-Rad liquid chip to detect fluorescence signal in the reaction system.The specificity of this method was evaluated by applying the proposed method to detect four viruses infecting potato，TBRV，Potato spindle tuber viroid (PSTVd)，Potato virus A (PVA) and Tomato spotted wilt virus (TSWV).The results showed that better specificity to TBRV and no reaction with other three viruses.The sensitivity of the method was 1 pg/μL of total RNA，which was equal to the conventional RT-PCR method.The successful detection of TBRV in potato samples suggested the feasibility of this procedure for routine testing.

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Last Update: 2014-08-22