Conservation in vitro and Establishment of the Propagation System on Male Sterile Lines of Marigold(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Title:: Conservation in vitro and Establishment of the Propagation System on Male Sterile Lines of Marigold

Author(s):: WU Ya-ping; TANG Dao-cheng; Center of Plateau Flower Research，Qinghai University,Xining,Qinghai 810016

Keywords:: marigold; male sterile line; lateral bud; culture medium formula; conservation in vitro

Abstract:: Lateral buds in nine male sterile lines of marigold were used as explants to screen their culture medium of induction，subculture and rooting in vitro.The results showed that the culture medium was suitable for lateral bud in male sterile lines of marigold in inducing cluster buds was MS+6-BA 0.3 mg/L+NAA 0.10 mg/L;The culture medium was suitable for subculture of cluster buds was MS+6-BA 0.5 mg/L+NAA 0.10 mg/L；the best culture medium for rooting was 1/2MS+IBA 0.10 mg/L.So far the concervation system in vitro of male sterile lines on marigold were preliminarily established.

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Last Update: 2014-08-22