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Study on Effect of Precultivation Period on Chromosome Doubling of Alocasia indica(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年01期
Page:
105-107
Research Field:
Publishing date:

Info

Title:
Study on Effect of Precultivation Period on Chromosome Doubling of Alocasia indica
Author(s):
CHEN Rong; ZHU Chang-san
Department of Eco-engineering，Guangxi Eco-engineering Vocational and Technical College，Liuzhou，Guangxi 545004
Keywords:
Alocasia indica; colchicine; polyploid
PACS:
S 681.9
DOI:
-
Abstract:
Explants of petiole were taken from in vitro grown plants and to induce polypoids through colchicine treatments.The results showed that tetraploid inductivity was different with different precultivation period and cocultivation period.In 12 cases including four different precultivation periods(0，3，6 and 9 days)and three different cocultivation periods(8，12 and 16 days)，inducing with petiole explants,preculturing in propagation medium without colchicine for 6 days and coculturing in progation medium with 120 mg/L colchicine for 16 days was the most efficient condition for tetraploid induction，yielding highiest inductivity(46.4%).Proplonging precultivation period，explants survial percentage and effective shoots per culture bottle increased gradually.

References:

[1]黄玉源,张施君.天南星科观赏植物重要品种及其繁育技术[J].仲恺农业技术学院学报,2002,15(4):54-59.

[2]杜宝贵,黄丽娟,张志胜,等.红掌花药培养[J].生物技术通报,2009(增刊):189-195.

[3]张志胜,黎扬辉,姜蕾,等.红掌四倍体的离体诱导及其鉴定[J].园艺学报,2007,34(3):729-734.

[4]李庆玲,陈荣.杨跃生,等.两种天南星科植物根尖组织染色体观察方法的优化[J].北方园艺,2012(2):134-136.

[5]张兴翠.花叶绿萝的多倍体诱导及快速繁殖[J].西南农业大学学报,2004,26(1):58-60.

[6]李涵,郑思乡,龙春林.齿瓣石斛多倍体的诱导初报[J].云南植物研究,2005,27(5):553-556.

[7]Nilanthi D，Chen X L，Zhao F C，et al.Induction of tetraploids from petiole explants through colchicine treatments in?Echinacea purpurea?L[J].J Biomed Biotech,2009,1155/2009/343485.

[8]Cai X，Kang X Y.In vitro tetraploid induction from leaf explants of Populus pseudo-simonii?Kitag[J].Plant Cell Rep,2011,30:1771-1778.


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Last Update: 2014-08-22