|Table of Contents|

Effect of Different Preservation Conditions on Pollen Viability of Amomum villosum  Lour.(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年10期
Page:
147-150
Research Field:
Publishing date:

Info

Title:
Effect of Different Preservation Conditions on Pollen Viability of Amomum villosum  Lour.
Author(s):
YU An-min1; 2; YANG Jin-fen1; 2; ZHAN Ruo-ting1; 2; CHEN Wei-wen1; 2
1.Research Center of Chinese Herbal Resource Science and Engineering，Guangzhou University of Traditional Chinese Medicine，Guangzhou,Guangdong 510006；
2.Key Laboratory of Chinese Medicine Resource from Lingnan(Guangzhou University of Chinese Medicine),Ministry of Education，Guangzhou,Guangdong 510006
Keywords:
A.villosum; pollen viability; preservation condition; pollen germination in vitro; MTT staining method
PACS:
S 567.23+9
DOI:
-
Abstract:
Taking Amomum villosum Lour. that cultivated in Guangzhou University of Chinese Medicine as material，the effect of different preservation conditions on the pollen viability of Amomum villosum Lour. were studied.The collected pollen were kept in different conditions for 0，3，6，9，12，24 and 48 hours respectively，then the pollen viability were determined using MTT staining method and in vitro germination method.The results showed the moderately high temperature and high humidity prolonged the life of pollen from A.villosum. In vitro germination method was appropriate to reveal the pollen viability.The optimum shortterm storage(≤9 h)condition for pollen of A.villosum was 27℃ high humidity，which was consistent with the growth habit of high temperature and humidity.

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Last Update: 2014-08-15