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Study on Factors Affecting Anther Culture in virto of Citrullus lanatus(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年10期
Page:
117-120
Research Field:
Publishing date:

Info

Title:
Study on Factors Affecting Anther Culture in virto of Citrullus lanatus
Author(s):
GOU Yan-xia1ZHANG Ming-fang2
1.College of Biochemical Engineering,Hohhot Vocational College,Hohhot,Inner Mongolia 010051;2.Department of Horticulture,Zhejiang University,Hangzhou,Zhejiang 310029
Keywords:
Citrullus lanatusanther culturecallus induction
PACS:
S 651
DOI:
-
Abstract:
Using three Citrullus lanatus cultivars of F1generation ‘Chunguang’,‘Nabite’,‘Xidu’ which including lots of merits as materials,the technology of anther culture were studied.The results showed that more callus were formed on MS+KT 1.5 mg/L+BA 2.0 mg/L+NAA 1.5 mg/L;the best concentration of sucrose for callus induction was 6%;the results indicated that the treating of 4℃,72 hours was the proper condition that make the frequencies of callus induction;the studying of culture conditions and culture means indicated that callus inducement was better when it was cultured at 30℃ for 72 h and then cultured at 25℃.The quality of watermelon callus induced in dark was much better than in light.

References:

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Memo

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Last Update: 2014-08-15