|Table of Contents|

Optimization for SSR-PCR Technology System in Cucurbita moschata(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年10期
Page:
103-106
Research Field:
Publishing date:

Info

Title:
Optimization for SSR-PCR Technology System in Cucurbita moschata
Author(s):
MENG Ling-jun1; LI Zhu-gang2; HAN Jun-yan2; WANG Xun2; LU Jie2; FU Yu-jie1
1.Engineering Research Center of Forest Bio Preparation，Ministry of Education，Northeast Forestry University，State Engineering Laboratory of Bio Resources Eco Utilization，Northeast Forestry University，Harbin，Heilongjiang 150040；
2.Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang，Heilongjiang Academy of Agriculture Sciences，Harbin，Heilongjiang 150086
Keywords:
Cucurbita moschata; SSR-PCR; orthogonal experiment; system optimization
PACS:
S 642.1
DOI:
-
Abstract:
Taking 3 dwarf inbred line and 2 sprawling inbred lines of Cueurbita moschata as materials,the specific SSR primers in Cucurbita moschata were designed using software Primer 5.0.The main conditions affecting SSR-PCR were optimized using orthogonal experiment and gradient PCR experiments so as to establish the optimal SSR-PCR reaction system.The results showed that 20 μL reaction system contained 1.5 mmol/L Mg2+，60 ng DNA，0.20 mmol/L dNTPs，0.25 mmol/L primer and 1.5 U Taq enzyme.The optimal annealing temperature was 44.1℃.

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Last Update: 2014-08-15