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Study on Optimization of Tissue Culture System of Sinningia speciosa  Benth. and  the Sensitivity on Kanamycin(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2013年10期
Page:
100-102
Research Field:
Publishing date:

Info

Title:
Study on Optimization of Tissue Culture System of Sinningia speciosa  Benth. and  the Sensitivity on Kanamycin
Author(s):
YIN Zhi-hui; SONG Li-li; SI Liang; YU Dian-si; GUO Chang-hong
Key laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang，College of Life Science and Technology，Harbin Normal University，Harbin，Heilongjiang 150025
Keywords:
Sinningia speciosa ;  Benth.; tissue culture; kanamycin
PACS:
S 682.2+9
DOI:
-
Abstract:
Taking the leaves of Sinningia speciosa  Benth. as explants，the concentration of the different hormone combinations of the Sinningia speciosa Benth. leaves regeneration system and the antibiotics sensitive to kanamycin were studied.The results showed that the significantly different effects of different hormone combinations induction of adventitious bud，the optimal medium for leaf induction was MS with 1.0 mg/L 6-BA and 0.1 mg/L NAA，while the 1/2 MS medium with 0.5 mg/L NAA was successful for rapid rooting.The leaves of Sinningia speciosa  Benth. with 200 mg/Lkanamycin were sensitive.

References:

[1]Zhang M Z，Ye D，Wang L L，et al.Overexpression of the cucumber LEAFY homolog CFL and hormone treatments alter flower development in gloxinia (Sinningia speciosa) [J].Plant Mol Biol,2008,67：419-427.
[2]祝清俊,周钟信,张宗江,等.大岩桐组织培养和快速繁殖[J].莱阳农学院学报，1995,12(1):47-49.
[3]徐全乐,谢亚红,刘文婷,等.大岩桐高频再生体系建立的两种途径[J].园艺学报,2010,37(1):135-140.
[4]唐伟斌,刘占牛.大岩桐组织培养与快繁技术研究[J].安徽农业科学,2005，33(8):1418.
[5]杨海燕,傅玉兰,黄新,等.大岩桐组织培养与快速繁殖[J].安徽农业科学,2006,34(5):896-897.
[6]周爱芳,李家洲.提高大岩桐组织培养和栽培技术措施的探索[J].广东轻工职业技术学院学报,2006,5(3):24-26.
[7]赵万苓,姜世平,付新生,等.利用农杆菌介导法将查尔酮合酶基因导入大岩桐[J].分子植物育种,2006,4(1):45-48.
[8]王海洪.大岩桐组织培养及其练苗移栽技术[J].北方园艺,2008(4)：232-233.
[9]徐全乐.大岩桐组织培养研究进展[J].北方园艺,2011(23):166-170.
[10]宋俊芳,张秋,罗明,等.绿色荧光蛋白基因转化大岩桐的研究[J].湖北大学学报(自然科学版),2001,23(2):171-173.
[11]胡鑫,徐全乐.连续组织培养引起大岩桐再生植株的表型变化[J].西北农业学报,2010,19(5):167-170.

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Last Update: 2014-08-15